Fig. 7

Exposure to multiple Chromobacterium species affects mortality of An. gambiae females. Five Chromobacterium species were cultured under biofilm conditions for 72 h and the biofilm and culture media (i.e. biofilm supernatant) were harvested and an aliquot was filtered through a 0.2 μm filter to remove live cells. Culture preparations, LB or PBS were mixed 1:1 with 3% sucrose and provided to An. gambiae adult females for 24 h. Experimental sugar meals were then removed, and all treatments were provided 10% sucrose ad libitum. Survival was monitored for seven days. The experiment was repeated three independent times, with 20–25 individuals per treatment per replicate. Only one PBS and LB control dataset was collected, and these data are repeated on each panel to allow visualization of each individual species. Contrasts between each treatment and the appropriate control (PBS for biofilm, LB for biofilm supernatant) were conducted using Log-Rank tests followed by a Bonferroni correction