Fig. 6

Effect of exposure to cell-free C.sp_P biofilm supernatant on transcript abundance in the midguts of adult female An. gambiae. Adult An. gambiae females were fed sugar meals containing either LB or 72 h filter-sterilized C.sp_P biofilm supernatant for 24 h, at which point midguts were dissected from 20 adult females per treatment in sterile 1× PBS and pooled in TRIzol. RNA from LB-fed females was co-hybridized with RNA from C.sp_P-fed females to a custom Agilent microarray. The experiment was repeated in full four independent times, and samples were co-hybridized within the same replicate experiment. The graph shows the number of genes from each functional category where transcript abundance significantly increased or decreased in response to cell-free C.sp_P biofilm supernatant exposure. a Genes from known functional categories. b Including genes with unknown function and diverse function. Abbreviations: UNK, unknown; DIV, diverse; R/T/T, replication, transcription, and translation; MET, metabolism; TRP, transport; C/S, cytoskeletal and structural; P/D, proteolysis and digestion; R/S/M, redox, stress-related, and mitochondrial; I/A, immunity and apoptosis