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Fig. 1 | Parasites & Vectors

Fig. 1

From: The mosquito adulticidal Chromobacterium sp. Panama causes transgenerational impacts on fitness parameters and elicits xenobiotic gene responses

Fig. 1

C.sp_P exposure causes mortality across a broad taxonomic range of disease vector mosquitoes. Planktonic overnight cultures of C.sp_P were washed two times with 1× PBS and diluted or concentrated to obtain “low” (i.e. approximately 105 or 106 CFU/ml) and “high” (i.e. approximately 1010 CFU/ml) bacterial cell densities, as per our previous work [17]. Bacteria of each density or 1× PBS as a control were then mixed 1:1 with 3% sucrose (final sucrose concentration 1.5%) and provided to adult females for 24 h, at which point all treatments were given 10% sucrose. a Aedes albopictus females: PBS vs C.sp_P 106, z = 1.93, P = 0.0531; PBS vs C.sp_P 1010, z = 6.67 P < 0.0001. b Culex quinquefasciatus: PBS vs C.sp_P 106, z = -1.06, P = 0.289; PBS vs C.sp_P 1010, z = 9.30, P < 0.0001. c Anopheles stephensi: PBS vs C.sp_P 105, z = 2.87, P = 0.004; PBS vs C.sp_P 1010, z = 10.26, P < 0.0001. a and c were repeated 3 times with 25–30 individuals per replicate, while b was repeated 5 times with 9–20 individuals per replicate. Survival curves were fitted using the Kaplan-Meier method with pooled data from all replicates. Vertical tick-marks indicate censored samples. Data from all experiments were analyzed using a Cox proportional hazards model including treatment and replicate

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