From: Recovering parasites from mummies and coprolites: an epidemiological approach
Method | Prelima | Quantb | Rehydratc | Disaggregd | Screeninge | Concentf | Post-analysisg |
---|---|---|---|---|---|---|---|
Callen | Cleaned, imaged, sectioned or cored | Lyco | 0.5% Na3PO4; 48hr | Magnetic stirrer, active separation of particles w/ water jet and spatula | 250 μm mesh | Centrifuge | Retain all macro and micro remains and unprocessed section |
Lutz, Korea | – | – | 0.5% Na3PO4; 1 wk | Agitation | Three layers of double gauze | Passive sediment 1 day then mixed w/10% neutral buffered formalin | – |
Lutz, Brazil | Cleaned | – | 0.5% Na3PO4 | Glass stir rod | Three layers of double gauze | Passive sediment 1 day | Retain all macro and micro remains and unprocessed section |
Reims | – | – | 0.5% Na3PO4 in 5% glycerin- water w/ formalin | Crushed then ultrasonic treatment | Screened w/315 mm, 160 mm, 50 mm, and 25 mm meshes. Micro remains retained on screen | – |