Fig. 4
Preparation of Sjpcdp10 dsRNA and analysis of the optimal RNAi conditions. a Agarose gel electrophoresis of Sjpcdp10 dsRNA. Lane PCDP10: Sjpcdp10 dsRNA; Lane D2000: markers; Lane EGFP: egfp dsRNA (negative control dsRNA). b Analysis of the optimal concentration of Sjpcdp10 dsRNA at the optimal RNAi time (6 days). Sjpcdp10 dsRNA concentrations: 0, 0.5, 1, 2, 4, 8, 16, 32, 64 × 10−5 mg/ml. c Analysis of the optimal incubation/soaking time with the optimal concentration of Sjpcdp10 dsRNA (4 × 10−5 mg/ml). RNAi incubations times with Sjpcdp10 dsRNA: 1 d, 2 d, 3 d, 4 d, 5 d, 6 d, 7 d