Fig. 2
From: Co-infections with multiple genotypes of Anaplasma marginale in cattle indicate pathogen diversity
qPCR amplification of A. marginale msp1β variants. a qPCR amplification of plasmid DNA (2.5 × 107 copies) of A. marginale msp1β variants (SA1, SA2, SA4, SA5, SA7). Genomic DNA (gDNA) from sample C14 was used as a positive control for A. marginale (A.m) and water as a negative control. gDNA from the A. centrale (A.c) vaccine strain, A. phagocytophilum (A.p), Anaplasma sp. (Omatjenne) (A.spO) and a no temple control (NTC) were included in the analysis. b Detection of tenfold serial dilutions (2.5 × 107 – 2.5 × 102 copies) of plasmid DNA of A. marginale variants SA2 and SA4. c Detection of tenfold serial dilutions of A. centrale vaccine strain (106–101 iRBCs) genomic DNA. Abbreviations: Cq, quantification cycle; R2, regression coefficient; E, assay efficiency