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Fig. 5 | Parasites & Vectors

Fig. 5

From: Culex pipiens pallens cuticular protein CPLCG5 participates in pyrethroid resistance by forming a rigid matrix

Fig. 5

Transmission electron microscopy (TEM) analysis of the ultrastructure of mosquito femur cuticles. a TEM image of the mosquito femur cuticle. Adults at the 3-day-old stage were injected with siCPLCG5 (500 ng per insect) or siNC (controls). The thickness of the cuticle of siCPLCG5-injected animals was thinner than that of the siNC group. b Gold labelling TEM of the mosquito femur cuticle. Ultrathin sections (~90 nm) were incubated with gold-labelled antibody (6 nm), and fewer gold particles were observed in siCPLCG5-injected animals than in siNC-injected animals. The blue bars indicate the exocuticle. c Column bar graph (vertical) of the exocuticle thickness. The graph shows the mean and SD for siNC (n = 6) and siCPLCG5 (n = 5) mosquito femurs. The thickness of the exocuticle of siNC group animals is 0.946 ± 0.126 μm, compared with 0.717 ± 0.110 μm for siCPLCG5 group (t-test, t(10) = 3.262, P = 0.009; **P < 0.01). Abbreviations: EN, endocuticle; EX, exocuticle. Scale-bars: a, 1 μm; b, 0.5 μm

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