Heat treatment and false-positive heartworm antigen testing in ex vivo parasites and dogs naturally infected by Dirofilaria repens and Angiostrongylus vasorum

Table 1 Assessment scores for the tests used in the study

Score	Witness®	SNAP® 4Dx® Plus	SNAP® HTWM	Speed Diro™	PetChek® HTWM PF Optical Reading NEG 0.0420+ 0.05 POS 0.6529	DiroCHEK® Optical Reading NEG 0.0562 POS 0.3754
0	No reaction	No reaction	No reaction	No reaction	≥ 0.0920	≥ 0.0562
+	Mild PR	Mild PR	1 spot less bright than control spot	1-3 into the Speed reading chart^a	≥ 0.0921 ≤ 0.37245	≥ 0.0563 ≤ 0.2158
++	Clear PR but less than control band/spot	Clear PR but less than control spot	1 spot as bright as control spot	4-7 into the Speed reading chart^a	≥ 0.37245 ≤ 0.6529	≥ 0.2159 ≤ 0.3754
+++	PR as clear as control band/spot	PR as clear as control band/spot	2 spots	8-10 into the Speed reading chart^a	≥0.6530	≥ 0.3755

^aColor chart provided by manufacturer and used to measure the intensity of the color change

ISSN: 1756-3305