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Table 1 Various methods of immune complex dissociation and the minimum starting volume of canine serum or plasma needed to recover 100 μL for the heartworm antigen ELISA test

From: Validation of immune complex dissociation methods for use with heartworm antigen tests

Reference

Method

Minimum volume of sample required to recover 100 μL for testing

Reagents added to the sample

Weil [5]

Heat + EDTA

100 μL

100 μL 0.1 M EDTA

IDEXX PetChek® HW antigen testa

Pepsin

50 μL

35 μL S1 (acid) + 35 μL S2 (base)

Pokriefka [13] HIV antigen test

Acidic pH

50 μL

35 μL S1 (acid) + 35 μL S2 (base)

Little [11]

Heat

600 μL

NA

  1. aThe sample is added to an enzyme-coated well of the microtiter plate from the PetChek® HW antigen test kit. Solutions S1 and S2 are reagents contained within the PetChek® HW antigen test kit and are added sequentially before transferring the mixture to the plate assay