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Fig. 3 | Parasites & Vectors

Fig. 3

From: Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors

Fig. 3

Representative gel for molecular detection of T. cruzi DNA in field triatomine samples by multiplex conventional PCR. Lane M: 100 bp molecular marker; Lanes 1, 2: PCR negative controls (PCR reagents without DNA and ultra-pure water, respectively); Lane 3: DNA extraction negative control (extraction reagents in ultrapure water); Lanes 4, 12: field triatomine samples; Lane 13: multiplex PCR positive control (R. prolixus midgut spiked with ~102 CL Brener epimastigotes); Lane 14: T. cruzi positive control (DNA extracted from 102 CL Brener epimastigotes); Lane 15: T. rangeli positive control (DNA extracted from 102 Macias strain epimastigotes)

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Parasites & Vectors

ISSN: 1756-3305

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