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Fig. 2 | Parasites & Vectors

Fig. 2

From: Development of a loop-mediated isothermal amplification technique and comparison with quantitative real-time PCR for the rapid visual detection of canine neosporosis

Fig. 2

LAMP and qPCR detection limits for 10-fold serial dilutions of N. caninum DNA. a Results of LAMP analysis. b Electrophoresis results of LAMP products. c Results of qPCR analysis. d N. caninum DNA standard curve and amplification efficiency. The crossing point values are plotted against the log of the initial template concentration (equivalent genomes of N. caninum DNA). e N. caninum DNA melting curves. Lane M: 100 bp DNA ladder; Lanes 1–7: 105, 104, 103, 102, 10 and 1 equivalent genomes of N. caninum DNA, respectively; Lane 7: negative control (sterile water)

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Parasites & Vectors

ISSN: 1756-3305

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