Fig. 1From: Development of a loop-mediated isothermal amplification technique and comparison with quantitative real-time PCR for the rapid visual detection of canine neosporosisSpecificities of the LAMP assay for the detection of N. caninum. a Specificity of the LAMP assay using HNB (the light blue colour indicates a positive sample). b Confirmation of the LAMP results using agarose gel (2%) electrophoresis. Lane M: 100Â bp DNA ladder; Lane 1: N. caninum; Lane 2: T. gondii; Lane 3: C. parvum; Lane 4: C. cayetanensis; Lane 5: G. duodenalis; Lane 6: E. histolytica; Lane 7: Entamoeba coli; Lane 8: Blastocystis; Lane 9: E. bieneusi; Lane 10: N. fowleri; Lane 11: Acanthamoeba spp.; Lane 12: Escherichia coli; Lane 13: blood from healthy heifer; Lane 14: blood from healthy dog; Lane 15: faeces from healthy dog; Lane 16: negative control (sterile water)Back to article page