Fig. 6

Hemolymph phenoloxidase activity of larvae and adults. a Time course of optical density (OD₄₉₀) measurements of hemolymph from naïve, injured, and E. coli-infected larvae, 1-day-old adults, and 5-day-old adults that was diluted in water containing L-DOPA. Data were analyzed by two-way ANOVA, followed by Šidák’s multiple comparisons post-hoc test. Regardless of treatment and time point, larvae showed higher phenoloxidase activity than 1-day-old and 5-day-old adults (Šidák’s: P ≤ 0.0263 for all comparisons). Whiskers denote the SEM. b Images of cuvette wells at the 30 min time point showing that melanization-induced darkening is more pronounced in the larval samples