Fig. 5

Inhibition of S. aureus -containing phagosome formation by the 14-3-3 inhibitor in C6/36 HT cells. C6/36 HT cells were treated with 14-3-3 inhibitor at different concentrations and evaluated for the ability to form phagosome with pHrodo green S. aureus bacteria. Again, phagosome formation (a, b) was significantly reduced by the 14-3-3 inhibitor in a dose-dependent manner. (c) Representative histograms of phagosome formation by phagosome formation by untreated C3/36 HT cells on ice (left, negative control), treated with 12.5 μM of 14-3-3 inhibitor (middle), and untreated C3/36 HT cells at 34 °C, in medium (right, positive control). Experiments were performed in triplicate and represented as the mean ± standard error, SE. Representative data from three independent experiments are shown