Skip to main content

Table 1 Summary of plasmid constructs used for genetic complementation of E. tenella

From: Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

Plasmid

Fluorescent reporter

Target gene

Gene promoter

p5Act-vvVP2a

5’EtMic1-mCitrine-3’EtMic1

vvVP2 (IBDV)

5’Et-Act

p5Act-gIa

5’EtMic1-mCitrine-3’EtMic1

gI (ILTV)

5’Et-Act

p5Act-mCheb

5’EtMic1-mCitrine-3’EtMic1

mCherry

5’Et-Act

p5TIF-vvVP2a

5’EtMic1-mCitrine-3’EtMic1

vvVP2 (IBDV)

5’Et-TIF

p5TIF-gIa

5’EtMic1-mCitrine-3’EtMic1

gI (ILTV)

5’Et-TIF

p5TIF-mCheb

5’EtMic1-mCitrine-3’EtMic1

mCherry

5’Et-TIF

  1. aUsed for the generation of stable populations
  2. bUsed to test mCherry expression in transient transfection