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Fig. 4 | Parasites & Vectors

Fig. 4

From: Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

Fig. 4

Humoral immune responses by chickens immunised with E. tenella transgenic populations and wild type parasites. a Proportion of chickens presenting a positive reaction against rec-vvVP2 or rec-gI by western blot. * Indicates significant differences between groups in the frequency of recombinant protein detection (P < 0.05, Fisher’s exact test). b Western blot of chicken sera against rec-vvVP2 (panel above); lanes 1-2: positive sera from chickens immunised with Et-Act-vvVP2-P6 at dilutions 1/50, 1/100 or 1/200; lanes 3-4: positive and negative sera from chickens immunised with Et-TIF-vvVP2-P2 at dilutions 1/50, 1/100 or 1/200; lane 5: negative control (sera from chickens immunised with E. tenella (wt) at dilution 1/50); lane 6: positive control (sera from chickens immunised with Vaxxitec at dilution 1/100) and Western blot of chicken sera against rec-gI (panel below); lanes 1–7: chicken reacting positive (1–3: Et-Act-gI-P5 at dilution 1/50; 4–7: Et-TIF-gI-P2 at dilution 1/50); lanes 8–12: chicken reacting negative (8–9: Et-Act-gI-P5 at dilution 1/50; 10–12: Et-TIF-gI-P2 at dilution 1/50); lane 13: negative control (sera from chickens immunised with E. tenella (wt) at dilution 1/50); lane 14: positive control (anti-chicken ILT Spafas at dilution 1/200). Rabbit anti-chicken IgG antibody HRP conjugate (Merk Millipore) at 1/1000 dilution was used as secondary antibody

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