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Fig. 3 | Parasites & Vectors

Fig. 3

From: Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

Fig. 3

Analysis of transgenic E. tenella populations. a The percentage fluorescence detected for each transgenic population with successive in vivo passage. b Number of transgene copies per parasite genome for each transgenic population with successive passage through chickens. The number of copies was calculated using a standard curve of E. tenella gDNA. c Western blot of the different transgenic populations; Et-Act-gI-P5: lanes 2, 5; Et-TIF-gI-P2: lanes 3, 6; Et-Act-vvVP2-P6: lanes 8, 11; Et-TIF-vvVP2-P2: lanes 9, 12; and E. tenella (wt): lanes 4, 7, 10, 13; incubated with MCA-LTV-Mab6-INT (lanes 2–4) or ILT Spafas (lanes 5–7) for gI recognition and MCA 10-INT (lanes 8–10) or IBDV R63 MoAb (lanes 11–13) for vvVP2 recognition. Lane 1 is the Mw. Solid black arrows indicate specific band recognition with a size of ~62 kDa for gI and a processed form of it ~56 kDa (lines 2 and 3). Dotted white arrows indicate potential band recognition with sizes of ~62 kDa for gI and ~42 kDa for vvVP2 (lines 5, 6 and 8)

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