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Fig. 5 | Parasites & Vectors

Fig. 5

From: Ecological scenario and Trypanosoma cruzi DTU characterization of a fatal acute Chagas disease case transmitted orally (Espírito Santo state, Brazil)

Fig. 5

Trypanosoma cruzi DTU molecular characterization of T. vitticeps isolates LBT 3198, LBT 3210 and LBT 3211. a Products amplified by the mini-exon gene and analyzed by electrophoresis on 2 % agarose gel stained with ethidium bromide. The sample LBT 3198 and LBT 3210 presented an amplicon size of 150 bp and was identified as zimodeme 3 (DTU TcIII-TcIV). The sample LBT 3211 was identified as TcI, as it presented an amplicon size of 200 bp. b PCR-RFLP with histone 3 gene/AluI restriction enzyme for T. cruzi DTU genotype on 3 % agarose gel stained with ethidium bromide. The samples LBT 3198 and LBT 3210 presented amplicons sizes of 210 bp and 440 bp, genotyping these samples as DTU TcIV. DTU reference strains: TcI-SylvioX/10cl1, TcII-Esmeraldocl3, TcIII-M5631cl5, TcIV-9212102R, TcV/TcVI-SC43cl1, T. rangeli-Choco, NC:negative control

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Parasites & Vectors

ISSN: 1756-3305

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