2-Cys peroxiredoxin is required in successful blood-feeding, reproduction, and antioxidant response in the hard tick Haemaphysalis longicornis

Table 2 Effects of HlPrx and/or HlPrx2 gene silencing in ticks

Knockdown groups	Infest No.	Drop No.	Engorged body weight (mg)	Egg weight (mg)	Ratio of egg weight/engorged body weight	Hatching rate (%)
dsLuc	30	25	263.7 ± 58.9	162.1 ± 38.9	0.61 ± 0.03	100
dsDouble	30	15	218.8 ± 66.2^a	130.3 ± 46.7^d	0.59 ± 0.08	87
dsHlPrx2	30	22	204.4 ± 56.3^b	116.7 ± 45.3^e	0.55 ± 0.09^g	77ⁱ
dsHlPrx	30	28	210.0 ± 59.8^c	124.8 ± 43.4^f	0.58 ± 0.06^h	78^j

^aSignificant difference as compared with the dsLuc group by Welch’s t-test: t ₍₂₇₎ = 3.16, P = 0.020
^bSignificant difference as compared with the dsLuc group by Welch’s t-test: t ₍₄₅₎ = 3.53, P = 0.001
^cSignificant difference as compared with the dsLuc group by Welch’s t-test: t ₍₅₀₎ = 3.24, P = 0.002
^dSignificant difference as compared with the dsLuc group by Welch’s t-test: t ₍₂₆₎ = 2.22, P = 0.036
^eSignificant difference as compared with the dsLuc group by Welch’s t-test: t ₍₄₂₎ = 3.67, P = 0.001
^fSignificant difference as compared with the dsLuc group by Welch’s t-test: t ₍₅₁₎ = 3.30, P = 0.002
^gSignificant difference as compared with the dsLuc group by Welch’s t-test: t ₍₂₅₎ = 2.85, P = 0.009
^hSignificant difference as compared with the dsLuc group by Welch’s t-test: t ₍₄₁₎ = 2.46, P = 0.018
ⁱSignificant difference as compared with the dsLuc group by Chi-square test: χ² = 6.36, df = 1, P = 0.012
^jSignificant difference as compared with the dsLuc group by Chi-square test: χ² = 6.04, df = 1, P = 0.014

ISSN: 1756-3305