Fig. 2

a Protein expression profiles of HlPrx2 in whole ticks during blood-feeding as analyzed by Western blot analysis. b Protein expression profiles of HlPrx2 in developmental stages. Arrow indicates native HlPrx2 protein as distinguished from the non-specific bands below. c Protein expression profiles of HlPrx2 in the internal organs (salivary glands, midgut, ovary, fat body, hemocytes, and synganglion). For loading control, tubulin was detected. The bars show the results of band densitometry analysis for HlPrx2. The relative expression was calculated based on tubulin. Data are presented as the mean ± standard deviation (SD). ^* P < 0.05, significant differences by Welch’s t-test. Abbreviations: Uf, unfed adults; Pf, partially fed adults at day 3; En, engorged adults