Fig. 1

Sequence alignment analysis of Eg-Grx1 and homologous glutaredoxins. The percentage homology of Eg-Grx1 with each glutaredoxin is shown at the end of the alignment. Regions of high identity and similarity between dithiol glutaredoxin sequences are shown as black and gray columns, respectively. The active-site residues CXXC are marked with a red box. The residues involved in interactions with the Gly and Cys of GSH are highlighted with yellow and green backgrounds. The residues interacting with ^γGlu of GSH are indicated by red letters. The predicted secondary structure of Eg-Grx1 is displayed above the alignment. Database accession numbers: Echinococcus granulosus glutaredoxin 1 (Eg-Grx-1) (GeneDB: EgrG_000124800), Echinococcus multilocularis glutaredoxin 1 (Em-Grx-1) (GenBank: CDI97471.1), Mus musculus glutaredoxin 1 (Mm-Grx-1) (GenBank: NP_444338.2), Homo sapiens glutaredoxin 1 (Hs-Grx-1) (GenBank: AAC35798.1), Plasmodium falciparum glutaredoxin 1 (Pf-Grx-1) (GenBank: BAB79691.1), Clonorchis sinensis glutaredoxin 3 (Cs-Grx-3) (GenBank: GAA51070.1), Caenorhabditis elegans glutaredoxin (Ce-Grx) (GenBank: NP_001040891.1), and Trypanosoma brucei glutaredoxin (Tb-Grx) (GenBank: CBV36792.1)