Fig. 2

Analysis of Ssλ15 expression and purification. a Coomassie Blue stained 10 % SDS-PAGE gel. Lane 1, cell-free extract from pGEX-Ssλ15 transformed E. coli BL21 cells loaded to the affinity column; Lane 2, GST- Ssλ15 fusion protein bound to the column; Lane 3, purified Ssλ15 sarcoptes moiety after cleavage with thrombin; Lane 4, GST bound to the column after cleavage; M, low molecular weight protein markers (Amersham Biosciences, Barcelona, Spain). The sample in Lane 3, separated by black lines, was resolved in a different gel from samples M, 1, 2 and 4. b-e Western blot analysis of GST-Ssλ15 protein using a serum sample from a rabbit experimentally-infested with S. scabiei (b), an anti-GST monoclonal antibody (c), a mix of sera from a non-infested rabbit and a non-infested chamois (d) and serum from a mange-infested chamois (e). Samples for Western blot analysis shown in b, c and d were resolved on the same gel, in a different gel from sample e, which was resolved in an independent gel