Identification of a novel β-adrenergic octopamine receptor-like gene (βAOR-like) and increased ATP-binding cassette B10 (ABCB10) expression in a Rhipicephalus microplus cell line derived from acaricide-resistant ticks

Table 1 Tick cell lines used in the study

Species	Cell line	Passage level tested	Instar of origin	Year initiated	Geographical origin (strain)	Reference	Acaricide resistance status of parent ticks if known
Rhipicephalus (Boophilus) microplus	BmVIII-SCC	41	Embryo	1979	Mexico	[32]	Susceptible
	BME/CTVM2	140	Embryo	1983	Costa Rica (Paquera)	[33]	Susceptible
	BME/CTVM5	67; 8	Embryo	1983	Colombia (Paso Ancho)	[20]	Resistant to organophosphates, organochlorines, Amitraz
	BME/CTVM6	211/233; 32; 221; 243	Embryo	1983	Colombia (Paso Ancho)	[33]	Resistant to organophosphates, organochlorines, Amitraz
	BME/CTVM23	54	Embryo	2005	Mozambique (Mozambique)	[34]	Not known
	BME/CTVM30	17	Embryo	2005	Mozambique (Mozambique)	[34, 35]	Not known
Rhipicephalus appendiculatus	RAN/CTVM3	66	Developing adult	1978	Kenya (Muguga)	[36]	Not known
Rhipicephalus appendiculatus	RA243	344	Developing adult	1971	East Africa	[37]	Not known
Rhipicephalus evertsi	REN/CTVM32	16	Developing adult	2010	South Africa	[23]	Not known
Rhipicephalus sanguineus	RML-RSE	83	Embryo	1980s	United States of America	[38, 39]	Not known
Rhipicephalus sanguineus	RSE/PILS35	4	Embryo	2012	France	This study	Not known
Amblyomma variegatum	AVL/CTVM13	127	Developing nymph	1989	Southern Africa	[40]	Not known
Hyalomma anatolicum	HAE/CTVM9	195	Embryo	1986	India (Ludhiana)	[41]	Not known
Ixodes ricinus	IRE/CTVM19	222	Embryo	1999	United Kingdom	[20]	Not known

All cell lines were grown in culture media and at incubation temperatures as described by Bell-Sakyi et al. [23] except RSE/PILS35, which was established in L-15 medium at 28 °C from eggs laid by a single incompletely-engorged female R. sanguineus tick kindly provided by Dr Cristina Socolovschi and Dr Oleg Mediannikov, URMITE, Marseille, France

ISSN: 1756-3305