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Fig. 3 | Parasites & Vectors

Fig. 3

From: Characterization of a midgut mucin-like glycoconjugate of Lutzomyia longipalpis with a potential role in Leishmania attachment

Fig. 3

GalNAc-containing glycoconjugates are sensitive to phosphatidylinositol-specific phospholipase C (PI-PLC) exposure. Lysate of Lu. longipalpis midguts was fractionated by Triton X-114 and analyzed by SDS-PAGE and western blot with lectin from Helix pomatia (HPA). HPA-binding molecules were detected mostly in the detergent phase (DET PLC-) and partially in the aqueous phase (AQ PLC-). In contrast, the HPA-binding molecule in midgut lysate after exposition to PI-PLC was detected mostly in the aqueous phase (AQ PLC+) and not in the detergent phase (DET PLC+)

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Parasites & Vectors

ISSN: 1756-3305

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