Skip to main content
Fig. 4 | Parasites & Vectors

Fig. 4

From: Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homolog

Fig. 4

Characterization of the kinase activity of TgCaMKrk. a In vitro kinase assays were performed using purified GST-TgCaMKrk and histone IIAS in the presence of EGTA or 0–10 μM Ca2+, or 10 μM calmodulin and 10 μM Ca2+. The autophosphorylation of TgCaMKrk was measured by scintillation counting. Effect of calmodulin antagonists on the (b) autophosphorylation and (c) histone IIAS phosphorylation activity of TgCaMKrk in the presence of DMSO or the calmodulin antagonists W-7 and calmidazolium (calmidz.) in a wheat germ cell-free system. The autophosphorylation and histone phosphorylation activity of TgCaMKrk were measured by scintillation counting. Representative data from three independent experiments are shown

Back to article page