Fig. 2From: Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homologExpression and purification of GST-GFP, GST-TgCaMKrk, and GST-TgCaMKrkKA. Proteins were expressed by using a wheat germ cell-free protein synthesis system. Total wheat germ extracts were subjected to affinity chromatography using glutathione-Sepharose beads. The purified proteins were separated on denaturing gels and then (a) silver stained or (b) transferred to a nitrocellulose sheet and probed with anti-GST antibodies. Lanes 1 and 2, GST-GFP; Lanes 3 and 4, GST-TgCaMKrk; Lanes 5 and 6, GST-TgCaMKrkKA. Lanes 1, 3, and 5, total wheat germ extracts; Lanes 2, 4, and 6, purified proteins. Molecular masses (kDa) are shown on the leftBack to article page