Table 1 Primers and annealing temperature for the identification of Enterocytozoon bieuensi
Gene locus | Primer sequences (5′–3′) | Annealing temperature (°C) | Expected product size (bp) | Reference |
|---|---|---|---|---|
ITS | F1: GATGGTCATAGGGATGAAGAGCTT, R1: AATACAGGATCACTTGGATCCGT | 55 | 410 | 21 |
F2: AGGGATGAAGAGCTTCGGCTCTG, R2: AATATCCCTAATACAGGATCACT | 55 | 392 | ||
MS1 | F1: CAAGTTGCAAGTTCAGTGTTTGAA, R1: GATGAATATGCATCCATTGATGTT | 58 | 843 | 22 |
F2: TTGTAAATCGACCAAATGTGCTAT, R2: GGACATAAACCACTAATTAATGTAAC | 58 | 676 | ||
MS3 | F1: CAAGCACTGTGGTTACTGTT, R1: AAGTTA GGGCATTTAATAAAATTA | 55 | 702 | 22 |
F2: GTTCAAGTAATTGATACCAGTCT, R2: CTCATTGAATCTAAATGTGTATAA | 55 | 537 | ||
MS4 | F1: GCATATCGTCTCATAGGAACA, R1: GTTCATGGTTATTAATTCCAGAA | 55 | 965 | 22 |
F2: CGA AGTGTACTACATGTCTCT, R2: GGACTTTAATAAGTTACCTATAGT | 55 | 885 | ||
MS7 | F1: GTTGATCGTCCAGATGGAATT, R1: GACTATCAGTATTACTGATTATAT | 55 | 684 | 22 |
F2: CAATAGTAAAGGAAGATGGTCA, R2: CGTCGCTTTGTTTCATAATCTT | 55 | 471 |