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Table 1 Ranges of parasite burden of Trypanosoma cruzi determined by real-time PCR in samples of Triatoma infestans used in xenodiagnosis (qPCR-XD) and peripheral blood (qPCR-B) in 100 individuals with chronic Chagas disease classified by positive or negative XD

From: Quantification by real-time PCR of Trypanosoma cruzi DNA in samples of Triatoma infestans used in xenodiagnosis of chronic Chagas disease patients

 

Positive XDa

Negative XDb

 

n = 21

n = 79

Ranges parasite burden (parasite equivalent/ml)

qPCR-XD†c

qPCR-B

qPCR-XD†c

qPCR-B

 

n (%)

n (%)

n (%)

n (%)

No Ct

0 (0)

0 (0)

13 (16.5)

17 (21.5)

0.1–1

0 (0)

8 (38.1)

27 (34.2)

29 (36.7)

1–10

2 (9.5)

7 (33.3)

32 (40.5)

30 (38.0)

10–100

5 (23.8)

4 (19.0)

5 (6.3)

2 (2.5)

100–1,000

9 (42.9)

1 (4.8)

2 (2.5)

1 (1.3)

1,000–10,000

5 (23.8)

1 (4.8)

0 (0)

0 (0)

  1. †Heteroscedasticity detected: Levene's test, F = 187.663, P = 0.0001
  2. aChi-square test (qPCR-XD vs qPCR-B): χ 2 = 91.82, df = 5, P = 0.0001
  3. bChi-square test (qPCR-XD vs qPCR-B): χ 2 = 6.71, df = 5, P = 0.1520
  4. cKruskal-Wallis test: χ 2 = 42.48, df = 2, P = 0.0001
  5. Abbreviation: No Ct, No threshold cycle (DNA-T.cruzi was not detected)