Fig. 1

PCR identification of T653K and M1 genes and recombinant plasmids pFastBac-T653K and pFastBac-M1 digested. The T. spiralis T653K gene was PCR-amplified from cDNA synthesized using a Prime Script 1^st Strain cDNA Synthesis Kit using total RNA extracted from T. spiralis Korean isotype (a). Influenza M1 gene was PCR amplified fromtotal RNA extracted from influenza virus (A/PR/8/34) (c). The T. spiralis T653K gene and influenza M1 gene were cloned into pFastBac with EcoRI/XhoI and SamI/XbaI enzymes, respectively, resulting in T653K plasmid (b) and M1 plasmid (d). Marker: DNA marker; size of T653K: 1,239 bp; size of M1: 1,027 bp