Fig. 6
Soaking Ae. aegypti (RJ strain) larvae with dsRNA-VGSCT6 increases pyrethroid (deltamethrin) susceptibility in adult mosquitoes of different ages. a Mortality percentage of 7, 15 and 30 day-old adult Ae. aegypti females from the RJ strain after 30 min exposure to deltamethrin pyrethroid (5 μg/ml). Adults were soaked in 0.5 μg/μl dsRNA in their previous 3rd instar larvae stage. Data represent the mean ± standard deviation of four treatment replicates, and 3 independent experiments were performed. Statistics: One-way Anova followed by Tukey’s multiple comparison test. b Adult females (three biological replicates with 5 mosquitoes each) previously soaked (0.5 μg/μl) with VGSCT6 dsRNA, Random dsRNA or only water (untreated) were analyzed by qRT-PCR for VGSC mRNA expression levels. Statistics: One-way Anova followed by Tukey’s multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.0005