Fig. 3
Soaking Ae. aegypti larvae with dsRNA-VGSC422 increases adult susceptibility to different concentrations of Deltamethrin. a Mortality percentage in 5 day-old Ae. aegypti adults from RJ strain (after dsRNA treatment in 3rd-instar larval stage - 0.5 μg/μl) at different Deltamethrin pyrethroid concentrations, after 30 min exposure. Data are shown as the mean ± standard deviation of four replicates, and 3 independent experiments were performed. Statistics: Two-way Anova followed by Bonferroni multiple comparison test. b Five day-old adult females (three replicates with 5 mosquitoes each) previously soaked with VGSC422 dsRNA, Random dsRNA or only water (untreated) were analyzed for VGSC mRNA expression levels using the Quantigene method. Final dsRNA soaking concentration: 0.5 μg/μl. Statistics: One-way Anova followed by Tukey’s multiple comparisons test. *P < 0.01, ***P < 0.0001. W: water (untreated); R: dsRNA random-treated; S: dsRNA VGSC422-treated