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Table 1 PCR primers used

From: Molecular identification of different trypanosome species and subspecies in tsetse flies of northern Nigeria

Target

Amplicon size (bp)

Forward primer nameb

Forward primer sequence (5’-3’)

Reverse primer nameb

Reverse primer sequence (5’-3’)

Reference

Trypanosome ITS1

250 to 700a

ITS1 CF

CCGGAAGTTCACCGATATTG

ITS1 BR

TTGCTGCGTTCTTCAACGAA

[9]

T. congolense Savannah-specific satellite DNA

316

TCS1

CGAGAACGGGCACTTTGCGA

TCS2

GGACAAACAAATCCCGCACA

[22]

T. congolense Forest-specific satellite DNA

350

TCF1

GGACACGCCAGAAGGTACTT

TCF2

GTTCTCGCACCAAATCCAAC

[22]

  1. aVarious sizes between 250 and 700 bp depending on the trypanosome(s) species present in the sample (T. brucei ssp.: ~480 bp; T. congolense Savannah/Forest: ~700 bp, T. congolense Kilifi ~620 bp; T. simiae: ~400 bp; T. simiae Tsavo: ~370 bp; T. godfreyi: ~300 bp and T. vivax: ~250 bp; bName used in the initial publication