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Table 3 Comparison between real-time PCR and LD RPA assays in detecting LD in 96 samples

From: Mobile suitcase laboratory for rapid detection of Leishmania donovani using recombinase polymerase amplification assay

Subjects

Sample type

Sensitivity (n/N)

Specificity (n/N)

Cases (N = 48)

 VL

Buffy coat

100 % (23/23)

NA

 Asymptomatic

100 % (5/5)

 PKDL

Skin biopsy

100 % (20/20)

Controls (N = 48)

 Endemic healthy control

Buffy coat

NA

100 % (35/35)

 Non-endemic healthy control

100 % (5/5)

 Disease control

100 % (8/8)

  1. Abbreviations: VL visceral leishmaniasis, PKDL post-kala-azar dermal leishmaniasis, n number of positives (for sensitivity) or negatives (for specificity), N total number of samples, NA not applicable