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Table 3 Comparison between real-time PCR and LD RPA assays in detecting LD in 96 samples

From: Mobile suitcase laboratory for rapid detection of Leishmania donovani using recombinase polymerase amplification assay

Subjects Sample type Sensitivity (n/N) Specificity (n/N)
Cases (N = 48)
 VL Buffy coat 100 % (23/23) NA
 Asymptomatic 100 % (5/5)
 PKDL Skin biopsy 100 % (20/20)
Controls (N = 48)
 Endemic healthy control Buffy coat NA 100 % (35/35)
 Non-endemic healthy control 100 % (5/5)
 Disease control 100 % (8/8)
  1. Abbreviations: VL visceral leishmaniasis, PKDL post-kala-azar dermal leishmaniasis, n number of positives (for sensitivity) or negatives (for specificity), N total number of samples, NA not applicable