Table 2 Cross-reactivity investigation for LD RPA assay
Pathogen name | Number of isolates | Sample type and reference test | LD RPA assay results | Source |
|---|---|---|---|---|
L. donovani MHOM/ET/67/HU3Z18 | 1 | Reference DNA with concentration of around 40–100 ng/μl | Positive | Paul-Ehrlich-Institute, Langen, Germany |
L. major MHOM/IL/81/FEBNI | 1 | Positive | ||
L. aethiopica MHOM/ET/72/L100Z14 | 1 | Positive | ||
L. infantum (50134D) MHOM/TN/80/IPT-1 | 1 | Positive | American Type Culture Collection, Manassas, USA | |
L. tropica (50129D) | 1 | Negative | ||
L. amazonensis | 1 | Negative | Dept. of Microbiology, University Medical Center Gottingen, Germany | |
L. braziliensis | 1 | Negative | ||
Toxoplasma gondii | 1 | Negative | ||
Plasmodium falciparum | 3 | DNA extracted from blood samples of malaria patients. Plasmodium species-specific nested PCR was performed [40]. | Negative | Icddr, b |
Plasmodium vivax | 3 | Negative | ||
Salmonella typhi | 1 | DNA extracted from culture | Negative | |
Mycobacterium tuberculosis | 2 | DNA extracted from lymph node aspirate from two extra pulmonary TB patients. Both were positive in culture and IS6110 PCR [41]. | Negative |