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Fig. 5 | Parasites & Vectors

Fig. 5

From: Pepsin is a positive regulator of Ac-cathB-2 involved in the rat gut penetration of Angiostrongylus cantonensis

Fig. 5

pH-dependence profile and hydrolysis activity of rAc-cathB-2. a pH-dependence profile of rAc-cathB-2 activity was measured with the specific substrate Z-RR-AMC. The data are presented as relative activities of Ac-cathB-2, where the highest activity at the pH optimum was taken as 100 %. b Digestive activity of rAc-cathB-2 on proteins of connective tissue components. After incubation for 6 h, all samples were fractioned in a 5 % gel followed by Coomassie brilliant blue staining. Lanes: 1, molecular weight markers; Type I collagen (Col), fibronectin (FN) and laminin (LMN) were incubated with PBS, Ac-cathB-2 or Ac-cathB-2 and E64 as indicated. c Influence of rAc-cathB-2 on isolated small intestine. After treating with rAc-cathB-2 for 2 h, the specimens were fixed and embedded in paraffin. Serial sections were stained with Mayer’s hematoxylin and eosin, and analysed under a light microscope. Scale-bar: 50 μm

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