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Fig. 5 | Parasites & Vectors

Fig. 5

From: Characterization of an epimastigote-stage-specific hemoglobin receptor of Trypanosoma congolense

Fig. 5

SPR assay of rTcHpHbR and rTbHpHbR. The quantitative binding assay of rTcHpHbR and rTbHpHbR was performed using an SPR assay. The vertical axis shows the binding response, while the horizontal axis shows the running time (in seconds). His-tagged rTcHpHbR or rTbHpHbR were used as immobilized receptor. a The interaction between rTbHpHbR and free-Hp (1), rTbHpHbR and free-Hb (2), rTcHpHbR and free-Hp (3), rTcHpHbR and free-Hb (4). The concentrations of used analytes were as follows: free-Hp 100 μg/ml (i), 10 μg/ml (ii), 1 μg/ml (iii) and free-Hb 100 μg/ml (iv), 10 μg/ml (v), 1 μg/ml (vi). b The interaction between the HpHb complex and rTbHpHbR (1 and 2) or rTcHpHbR (3 and 4). The concentrations of used analytes were as follows: Hp 100 μg/ml and Hb 50 μg/ml (i), Hp 50 μg/ml and Hb 50 μg/ml (ii and v), Hp 10 μg/ml and Hb 50 μg/ml (iii), Hp 50 μg/ml and Hb 100 μg/ml (iv), Hp 50 μg/ml and Hb 10 μg/ml (vi)

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