Fig. 4
From: Characterization of an epimastigote-stage-specific hemoglobin receptor of Trypanosoma congolense
Assessment of qualitative binding between rTcHpHbR and hemoglobin by a pull-down assay. The qualitative interaction between rTcHpHbR and free-Hb was analyzed by a pull-down assay using glutathione sepharose beads. Free-Hb (Lane 1), GST-rTcHpHbR (Lane 4) and GST (Lane 7) were used as the size standards of each protein. GST-rTcHpHbR (Lanes 2–4) and GST (Lanes 5–7) were used as bait proteins. Free-Hb (Lanes 2, 5) and diluted FBS (Lanes 3, 6) were used as prey proteins