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Fig. 2 | Parasites & Vectors

Fig. 2

From: Characterization of an epimastigote-stage-specific hemoglobin receptor of Trypanosoma congolense

Fig. 2

Expression profile of TcHpHbR. a The TcHpHbR mRNA expression profile was examined by Northern blotting. Total RNA extracted from TcIL3000 BSF (Lane 1), PCF (Lane 2), EMF (Lane 3) and MCF (Lane 4) were used for the analysis. The DNA probe to detect TcHpHbR mRNA was prepared by a PCR using the primers shown in Table 1. 18S rRNA was used as an internal reference. b The TcHpHbR protein expression profile was examined by Western blotting. Total proteins (400 μg/ml) extracted from TcIL3000 BSF (Lane 1), PCF (Lane 2), EMF (Lane 3) and MCF (Lane 4) were analyzed by immunoblotting using anti-rTcHpHbR mouse sera. c The cellular localization of TcHpHbR in TcIL3000 BSF (1), PCF (2), EMF (3) and MCF (4) was examined in an indirect immunofluorescence assay (IFA) using anti-rTcHpHbR mouse sera. The green, blue and red signals indicate TcHpHbR, DNA (bold arrow: nucleus, fine arrow: kinetoplast) and the endocytic compartment (arrowhead), respectively

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