An antibody against an Anopheles albimanus midgut myosin reduces Plasmodium berghei oocyst development

Parasites & Vectors

Table 2 Anopheles albimanus midgut proteins that were immunoprecipitated using mAb A-140 and identified by LC-MS/MS

Protein name	Accession No.	Mascot score	M_r (kDa)	Signal P*	Features
Myosin heavy chain	AALB007909-PA	6197	250	N	Myosin head motor domain
Hypothetical protein	AALB001083-PA	994	153	Y	Insulin-like growth factor binding protein domain, N-terminal domain/4 N-linked glycosylation sites/41 O-linked glycosylation sites
Protein lingerer	AALB004761-PA	108	154	N	Gly-Ala rich N-terminal domain/Ser-Gly-Ala-Gln rich C-terminal domain
Apaf-1 like killer protein (ARK)	AALB010165-PA	41	164	N	N-terminal death domain/P-loop Nucleoside triphosphate hydrolase/C-terminal WD-40 repeat domains
Hypothetical protein	AALB005141-PA	39	192	N	Calcium binding domain/Kazal-type serine protease inhibitor domain/Thyroglobulin Type 1 domain

*Prediction algorithms: SignalP 3.0was used to predict the presence of a signal peptide (http://www.cbs.dtu.dk/services/SignalP/). NetNGlyc 1.0 and NetOGlyc 4.0 cut-off scores (>0.7 threshold) (http://www.cbs.dtu.dk/services/). GlycoEP (http://www.imtech.res.in/raghava/glycoep/index.html) was also used to predict N- and O-linked glycan modification sites. The number of sites in common between NetNGlyc/NetOGlyc and GlycoEP are indicated in the table above

ISSN: 1756-3305