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Table 3 Real-time PCR detection and quantification of Leishmania infantum and Anaplasma platys DNA. in tissue samples from pregnant bitches. Starting Quantity (SQ) mean value of parasite load per PCR reaction

From: Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation

Dogs

Blood

Bone marrow

Skin

Uterus

Ovary

Placentae

L. infantum (SQ mean)

A. platys (SQ mean)

L. infantum (SQ mean)

A. platys (SQ mean)

L. infantum (SQ mean)

A. platys (SQ mean)

L. infantum (SQ mean)

A. platys (SQ mean)

L. infantum (SQ mean)

A. platys (SQ mean)

L. infantum (SQ mean)

A. platys (SQ mean)

820634

–b

2.31 × 10-1

–

3.50 × 10-2

2.36 × 10-3

–

–

2.78 × 10-2

–

–

–

–

821717

–

–

2.68 × 10-3

–

1.50 × 10-3

–

–

–

–

–

–

6.2 × 10-3

828965

–

–

2.61 × 10-3

3.36 × 102

5.70 × 10-3

3.52 × 10

–

2.75 × 10-2

–

–

–

–

821240

–

3.85 × 10-2

2.08 × 10-3

2.31 × 10-1

8.28 × 10-3

3.75 × 10-2

–

–

–

2.27 × 10-2

1.21 × 10-3

–

823993

–

–

3.43 × 10-3

–

3.57 × 10-3

–

–

–

–

–

–

–

42686

1.40 × 10-3

–

6.14 × 10-4

–

5.54 × 10-3

–

–

–

–

–

–

–

43077

–

1.68 × 10-1

–

2.91 × 10-1

3.79 × 10-3

–

–

1.88 × 10-1

–

2.73 × 10-1

–

–

42783

–

–

1.52 × 10-3

–

9.02 × 10-3

2.83 × 10-2

–

–

–

–

–

–

43055

–

–

2.70 × 10-4

–

5.88 × 10-4

–

–

–

–

–

–

–

42825a

–

–

–

–

–

–

–

–

–

–

–

–

  1. aNegative control; bqPCR negative for pathogen DNA