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Table 1 Oligonucleotide primer pairs used in PCR amplifications for the detection of Theileria equi and Babesia caballi in equines

From: Assessment of Theileria equi and Babesia caballi infections in equine populations in Egypt by molecular, serological and hematological approaches

Parasite

Primer name

Gene name

PCR reaction

Amplicon size

Primer sequence

Reference

T. equi

Beq-F

ema-1

External

567 bp

5'-GAG GAG GAG AAA CCC AAG-3'

Baptista et al [36]

Beq-R

   

5'-GCC ATC GCC CTT GTA GAG-3'

 

BeqN-F

 

Nested

229 bp

5'-TCA AGG ACA ACA AGC CAT AC-3'

 

BeqN-R

   

5'-TTG CCT GGA GCC TTG AAG-3'

 

B. caballi

Bca-F

rap-1

External

375 bp

5'-GATTACTTGTCGGCTGTGTCT-3'

Schwint et al [37]

Bca-R

   

5'-CGCAAGTTCTCAATGTCAG-3'

 

BcaN-F

 

Nested

224 bp

5'-GCTAAGTACCAACCGCTGA-3'

 

BcaN-R

   

5'-CGCAAGTTCTCAATGTCAG-3'

 
  1. The primer sets used for the primary reaction were: Beq-F and Beq-R for the amplification of the ema-1 T. equi gene, and Bca-F and Bca-R for the amplification of the rap-1 B. caballi gene. The primer sets used for nested PCR reaction were: BeqN-F and BeqN-R for the amplification of the ema-1 T. equi gene, and BcaN-F and BcaN-R for the amplification of the rap-1 B. caballi gene