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Fig. 4 | Parasites & Vectors

Fig. 4

From: Dietary phytochemicals modulate skin gene expression profiles and result in reduced lice counts after experimental infection in Atlantic salmon

Fig. 4

Relative gene expression analysed by qPCR and microarray (MA) in skin behind dorsal fin from four groups of L. salmonis-infected Atlantic salmon fed different diets; not-infected high dose (NI-HD), infected control (I-C), infected low dose (I-LD) and infected high dose (I-HD). Relative gene expression is presented as ± fold difference. Bars represent mean fold ± SEM compared to not-infected control fish, NI-C. Number of fish in each qPCR group is 9 and 5 in MA analysis. One-way ANOVA followed by post-hoc Tukey’s multiple comparisons tests was performed between groups and control. Asterisks above bars denote significant differences between groups and control: ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05. Joined brackets show significant differences between experimental groups. The average correlation coefficient between MA and qPCR data was 0.8. a Relative gene expression of barrier-to-autointegration factor 1 (BANF), CXCL10, leukocyte cell derived chemotaxin 2 (LECT2), Zymogen granule membrane protein 16 (ZG16) and Cathelicidin-derived antimicrobial peptide 2 (Cathelicidin) . b Relative gene expression analysed by qPCR of interleukin 4/13 (IL4/13), interleukin 17A (IL17A), interleukin 8 (IL8), and interferon gamma (IFNγ). c Relative gene expression analysed by qPCR of complement C3 (C3) and myeloperoxidase (MPO)

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