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Fig. 1 | Parasites & Vectors

Fig. 1

From: Real-time PCR using FRET technology for Old World cutaneous leishmaniasis species differentiation

Fig. 1

a Primer and fluorescence probe positions selected for FRET-based real-time PCR of Leishmania cathepsin L-like cysteine protease B gene. Sequences of forward (cpb F) and reverse (cpb R) primers were aligned with the corresponding target sequences. Forward primer harbors one wobble base (R = A/G). FRET hybridization probes (cpb sensor 2 and cpb anchor 2) were both designed antisense for detection of parasite. Sensor FRET hybridization probe was designed to be specific for cathepsin L-like cysteine protease B gene of L. tropica (GenBank Accession number: DQ286773) with one nucleotide mismatch difference from that of L. major (GenBank Accession number: AJ512654) and two nucleotide mismatch differences from that of L. aethiopica (GenBank Accession number: DQ071678). b Alignment of the OWCL species and species causing visceral and mucocutaneous leishmaniasis. Sequences are colour-coded by percentage identity

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