Fig. 3
Effect of in culture inhibition of TbPARP on parasite growth. PAR formation was assessed by Dot Blot (a) in procyclic cultures pre-incubated for 30 min with PARP inhibitors and treated with 500 μM H2O2 for 10 min, and (b) in bloodstream cultures pre-incubated for 10 min with PARP inhibitors and treated with 250 μM H2O2. Positive controls (H2O2) correspond to those reactions with no inhibitors added, while negative controls (control) correspond to parasites not treated with H2O2. The membranes were stained with Ponceau Red as a loading control (LC). Lower panels in both cases show the intensity ratio of PAR to LC signals calculated using ImageJ software. c Effect of the selected PARP inhibitors on procyclic parasites’ growth after 48 h incubation. d Effect of the selected PARP inhibitors on bloodstream parasites’ growth after 24 h incubation. Nifurtimox was used at 2.5 μM and inhibitors were used at the same concentrations as in panel B. Culture growth in absence of inhibitors or Nifurtimox was considered as 100 %. Triplicates were performed for every data point, and expressed as means and standard deviations. Statistical significance is specified in comparison to control groups (***, P < 0.001; **, P < 0.01; *, P < 0.05)