Table 1 Primers and probes used in tick species determination and pathogen screening

qPCR:

 IXO-I2-F4

Ixodes spp. ITS2

TCTCGTGGCGTTGATTTGC

This paper

 IXO-I2-R4

Ixodes spp. ITS2

CTGACGGAAGGCTACGACG

 Ipe-I2-P4

I. persulcatus ITS2

[FAM]-TGCGTGGAAAGAAAACGAG-[BHQ1]

 Iri-I2-P4

I. ricinus ITS2

[VIC]-TGCTCGAAGGAGAGAACGA-[BHQ1]

 Bb23Sf

B. burgdorferi 23S RNA

CGAGTCTTAAAAGGGCGATTTAGT

Courtney et al. [83]

 Bb23Sr

B. burgdorferi 23S RNA

GCTTCAGCCTGGCCATAAATAG

 Bb23Sp

B. burgdorferi 23S RNA

[FAM]-AGATGTGGTAGACCCGAAGCCGAGTG-[BHQ1]

 Bmi-F

B. miyamotoi glpQ

CACGACCCAGAAATTGACACA

Vayssier-Taussat et al. [84]

 Bmi-R

B. miyamotoi glpQ

GTGTGAAGTCAGTGGCGTAAT

 Bmi-P

B. miyamotoi glpQ

[FAM]-TCGTCCGTTTTCTCTAGCTCGATTGGG-[BHQ1]

 Bart-ssRA-F

Bartonella ssRa

GCTATGGTAATAAATGGACAATGAAATAA

Diaz et al. [85]

 Bart-ssRA-R

Bartonella ssRa

GCTTCTGTTGCCAGGTG

 Bart-ssRA-P

Bartonella ssRa

[FAM]-ACCCCGCTTAAACCTGCGACG-[BHQ1]

 Rspp-F

Rickettsia gltA

GAGAGAAAATTATATCCAAATGTTGAT

Labruna et al. [86]

 Rspp-R

Rickettsia gltA

AGGGTCTTCGTGCATTTCTT

 Rspp-P

Rickettsia gltA

[CY5]-CATTGTGCCATCCAGCCTACGGT-[BHQ3]

 CNe-F

Ca. N. mikurensis groEL

AGAGACATCATTCGCATTTTGGA

Vayssier-Taussat et al. [84]

 CNe-R

Ca. N. mikurensis groEL

TTCCGGTGTACCATAAGGCTT

 CNe-P

Ca. N. mikurensis groEL

[TAMRA]-AGATGCTGTTGGATGTACTGCTGGACC-[BHQ2]

PCR:

 CS877f

Rickettsia gltA

TAATACGACTCACTATAGGGGGGGACCTGCTCACGGCGG

Mediannikov et al. [87]

 CS1258r

Rickettsia gltA

ATTAACCCTCACTAAAGATTGCAAAAAGTACAGTGAACA