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Fig. 4 | Parasites & Vectors

Fig. 4

From: RNAi-mediated gene knockdown by microinjection in the model entomopathogenic nematode Heterorhabditis bacteriophora

Fig. 4

RNAi mediated phenotype and transcript changes in H. bacteriophora injected with dpy-7 dsRNA. Adult H. bacteriophora hermaphrodites that were injected with dpy-7 dsRNA produced progeny with dumpy phenotype. a. Phenotypically wild type progeny of H. bacteriophora worms injected with dpy-7 dsRNA. b. Progeny of H. bacteriophora worms injected with dpy-7 dsRNA exhibiting the dumpy phenotype. c. Expression of dpy-7 gene in the progeny of dpy-7 dsRNA injected worms. The y-axis represents the fold change in mRNA expression in the progeny of H. bacteriophora hermaphrodites. The mRNA levels are normalized to dpy-7 expression in the progeny of non-injected hermaphrodites (black bar). The green bar represents dpy-7 levels in progeny of gfp injected worms. The red bar represents dpy-7 levels in phenotypically wild type siblings, and the blue bar represents the dpy-7 transcript levels in phenotypically dumpy worms. To control for off-target effects, expression of an unrelated gene (dpy-13) in dumpy worms was determined (brown bar). The graph was produced by combining data from at least three independent biological replicates. Error bars indicate the standard error of the mean. Asterisks depict the statistical significance of the observed differences in unpaired, two-tailed t-tests with P-values < 0.01 (*) and < 0.001 (**)

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