Fig. 2

RNAi mediated phenotype and transcript changes in H. bacteriophora injected with nol-5 dsRNA. Adult H. bacteriophora hermaphrodites that were injected with nol-5 dsRNA produced progeny with no germline and empty gonad. a. Progeny of non-injected H. bacteriophora. The area with white dotted lines indicates the position of the gonads. b. Progeny of H. bacteriophora injected with nol-5dsRNA. c. Expression of nol-5 gene in the progeny of nol-5 dsRNA injected worms. The y-axis represents the fold change in mRNA expression in the progeny of H. bacteriophora hermaphrodites. The mRNA levels are normalized to nol-5 expression in the progeny of non-injected hermaphrodites (black bar). The green bar represents nol-5 levels in progeny of gfp injected worms. The red bar represents nol-5 levels in phenotypically wild type siblings, and the blue bar represents the nol-5 levels in worms with empty gonads. To control for off-target effects, expression of an unrelated gene (cct-2) in worms with empty gonads was determined (brown bar). The graph was obtained by combining data from at least three independent biological replicates. Error bars indicate the standard error of the mean. Asterisks depict the statistical significance of the observed differences in unpaired, two-tailed t-tests with P-values < 0.001 (**)