Fig. 1

RNAi mediated phenotype and transcript changes in H. bacteriophora injected with cct-2 dsRNA. Adult H. bacteriophora hermaphrodites that were injected with cct-2 dsRNA produced progeny with no germline and empty gonad. a. Progeny of non-injected H. bacteriophora. The area with white dotted lines indicates the position of the gonads. b. Progeny of H. bacteriophora injected with cct-2 dsRNA. c. Expression of cct-2 gene in the progeny of injected worms. The y-axis represents the fold change in mRNA expression in the progeny of H. bacteriophora hermaphrodites. The mRNA levels are normalized to cct-2 expression in the progeny of non-injected hermaphrodites (black bar). The green bar represents cct-2 levels in progeny of gfp injected worms. The red bar represents cct-2 levels in a phenotypically wild type sibling, and the blue bar represents the cct-2 levels in worms with empty gonads. To control for off-target effects, expression of an unrelated gene (nol-5) in worms with empty gonads was determined (brown bar). The graph was obtained by combining data from at least three independent biological replicates. Error bars indicate the standard error of the mean. Asterisks depict the statistical significance of the observed differences in unpaired, two-tailed t-tests with P-values < 0.001 (**)