Fig. 1
From: Flow cytometry analysis of the microbiota associated with the midguts of vector mosquitoes
SYBR Green I (SGI) serial dilution to determine optimum dilution rate for discrimination between cells and debris and between live and dead microbiota in the mosquito midgut. Each scattergram represent flow cytometric dot plots of red (FL3) versus green (FL1) fluorescence of midgut homogenate suspension stained concurrently with different SGI dilution with respect to a fixed PI at fixed concentration (12 μM) as recommended by the manufacturer. Four regions in the scattergram (A, B, C, D) represent different population of FCM collection, i.e. (A) PI intensive cells representing dead cells, (B) both PI and SGI positive cells representing bacterial cells with partially compromised membrane, (C) background noise and autofluorescing debris and (D) SGI intensive cells representing live bacteria. The number at the center of the plot correspond to the ratio of mean fluorescent intensity (±95 % CI) between bright and dim bacterial cell populations on the SYBR channel