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Fig. 3 | Parasites & Vectors

Fig. 3

From: Evaluation of recombinant multi-epitope proteins for diagnosis of goat schistosomiasis by enzyme-linked immunosorbent assay

Fig. 3

Expression and purification analysis of recombinant proteins. a, rBSjRAD23-1; b, rBSj23; c, rBSjPGM-BSjRAD23-1; d, rBSjPGM-BSj23; e, rBSjPGM-BSjRAD23-1-BSj23; f, rBSjRAD23-2-BSjPGM-BSj23. Lane M: Molecular markers; Lane 1: Total extract from vectors pET-28a (+) (B: pET-32a (+) vector with no IPTG) after induction with 1 mM IPTG; Lane 2: Total extract from recombination proteins after induction with 1 mM IPTG; Lane 3: The recombinant protein purified through Ni2+-charged column chromatography; Lane 4: Total extract from pET-32a (+) vector; Lane 5: Total extract from from pET-32a (+) vector after induction with 1 mM IPTG

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